Biotechnology Principles and Processes
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Biotechnology Principles and Processes
108 questions · Biology · NEET
- In the above represented plasmid, an alien piece of DNA is inserted at EcoRI site. Which of the following strategies will be chosen to select the recombinant colonies? Includes diagram2025 · Q1 · MCQ
- Polymerase chain reaction (PCR) amplifies DNA following the equation.2025 · Q18 · MCQ
- Identify the part of a bio-reactor which is used as a foam braker from the given figure. Includes diagram2025 · Q22 · MCQ
- The blue and white selectable markers have been developed which differentiate recombinant colonies from nonrecombinant colonies on the basis of their ability to produce colour in the presence of a chromogenic substrate. Given below are two…2025 · Q57 · MCQ
- Silencing of specific mRNA is possible via RNAi because of2025 · Q59 · MCQ
- Which of the following enzyme(s) are NOT essential for gene cloning? A. Restriction enzymes B. DNA ligase C. DNA mutase D. DNA recombinase E. DNA polymerase Choose the correct answer from the options given below:2025 · Q70 · MCQ
- Given below are two statements: Statement 1 : The DNA fragments extracted from gel electrophoresis can be used in construction of recombinant DNA. Statement II : Smaller size DNA fragments are observed near anode while larger fragments are…2025 · Q83 · MCQ
- Hind II always cuts DNA molecules at a particular point called recognition sequence and it consists of:2024 · Q3 · MCQ
- The "Ti plasmid" of Agrobacterium tumefaciens stands for2024 · Q67 · MCQ
- Given below are two statements: Statement I : The Indian Government has set up GEAC, which will make decisions regarding the validity of GM research. Statement II : Biopiracy is the term used to refer to the use of bio-resources by native…2024 · Q11 · MCQ
- Match List-I with List - II : Choose the correct answer from the options given below: Includes table2024 · Q22 · MCQ
- Identify the incorrect statement related to gel electrophoresis.2024 · Q34 · MCQ
- Recombinant DNA molecule can be created normally by cutting the vector DNA and source DNA respectively with:2024 · Q42 · MCQ
- The Bt toxin in genetically engineered Bt cotton kills the pest by:2024 · Q44 · MCQ
- Select the restriction endonuclease enzymes whose restriction sites are present for the tetracycline resistance (tet) gene in the pBR322 cloning vector.2024 · Q62 · MCQ
- Which of the following are correct about EcoRI? A. Cut the DNA with blunt end B. Cut the DNA with sticky end C. Recognise a specific palindromic sequence D. Cut the DNA between the base and when encounters the DNA…2024 · Q73 · MCQ
- Following are the steps involved in the process of PCR. A. Annealing B. Amplification (~1 billion times) C. Denaturation D. Treatment with Taq polymerase and deoxynucleotides E. Extension Choose the correct sequence of steps of PCR from…2024 · Q90 · MCQ
- Thermostable DNA polymerase used in PCR was isolated from :2023 · Q22 · MCQ
- Ligation of foreign DNA at which of the following site will result in loss of tetracyclin resistance of pBR322 :2023 · Q26 · MCQ
- Match List I with List II. Choose the correct answer from the options given below : Includes table2023 · Q37 · MCQ
- Which of the following is not a cloning vector?2023 · Q53 · MCQ
- Given below are two statements: one is labelled as Assertion (A) and the other is labelled as Reason (R). Assertion (A) : When a particular restriction enzyme cuts strand of DNA, overhanging stretches or sticky ends are formed. Reason (R)…2022 · Q3 · MCQ
- Separation of DNA fragments is done by a technique known as2022 · Q31 · MCQ
- The enzyme (a) is needed for isolating genetic material from plant cells and enzyme (b) for isolating genetic material from fungus. Choose the correct pair of options from the following :2022 · Q42 · MCQ
- Match List-I with List-II : Choose the correct answer from the options given below: Includes table2022 · Q47 · MCQ
- Pathogenic bacteria gain resistance to antibiotics due to changes in their :2022 · Q61 · MCQ
- Which of the following methods is not commonly used for introducing foreign DNA into the plant cell?2022 · Q79 · MCQ
- Refer to the following statements for agarose-gel electrophoresis: (a) Agarose is a natural polymer obtained from sea-weed. (b) The separation of DNA molecules in agarose-gel electrophoresis depends on the size of DNA. (c) The DNA migrates…2022 · Q93 · MCQ
- Given below are two statements : one is labelled as Assertion (A) and the other is labelled as Reason (R). Assertion (A) : Polymerase chain reaction is used in DNA amplification. Reason (R) : The ampicillin resistant gene is used as a…2022 · Q17 · MCQ
- Which one of the following statement is not true regarding gel electrophoresis technique?2022 · Q26 · MCQ
- Given below are two statements: Statement I : Restriction endonucleases recognise specific sequence to cut DNA known as palindromic nucleotide sequence. Statement II : Restriction endonucleases cut the DNA strand a little away from the…2022 · Q51 · MCQ
- Which of the following is not a desirable feature of a cloning vector?2022 · Q83 · MCQ
- Match List-I with List-II . Choose the correct answer from the options given below. Includes table2021 · Q22 · MCQ
- Which of the following is not an application of PCR (Polymerase Chain Reaction)?2021 · Q29 · MCQ
- Which of the following is a correct sequence of steps in a PCR (Polymerase Chain Reaction)?2021 · Q30 · MCQ
- Plasmid pBR322 has Pst I restriction enzyme site within gene ampR that confers ampicillin resistance. If this enzyme is used for inserting a gene for -galactoside production and the recombinant plasmid is inserted in an E.coli strain2021 · Q43 · MCQ
- During the process of gene amplification using PCR, if very high temperature is not maintained in the beginning, then which of the following steps of PCR will be affected first?2021 · Q58 · MCQ
- A specific recognition sequence identified by endonucleases to make cuts at specific positions within the DNA is :2021 · Q67 · MCQ
- During the purification process for recombinant DNA technology, addition of chilled ethanol precipitates out :2021 · Q9 · MCQ
- In gel electrophoresis, separated DNA fragments can be visualized with the help of2020 · Q59 · MCQ
- Bt cotton variety that was developed by the introduction of toxin gene of Bacillus thuringiensis (Bt) is resistant to2020 · Q60 · MCQ
- Identify the wrong statement with regard to Restriction Enzymes2020 · Q61 · MCQ
- The specific palindromic sequence which is recognized by EcoRl is2020 · Q62 · MCQ
- The sequence that controls the copy number of the linked DNA in the vector, is termed2020 · Q63 · MCQ
- Match the organism with its use in biotechnology Select the correct option from the following: Includes table2020 · Q64 · MCQ
- Choose the correct pair from the following2020 · Q76 · MCQ
- DNA precipitation out of a mixture of bimolecules can be achieved by treatment with :2019 · Q48 · MCQ
- Following statements describe the characteristics of the enzyme Restriction Endonuclease. Identify the incorrect statement.2019 · Q50 · MCQ
- The correct order of steps in Polymerase Chain Reaction (PCR) is2018 · Q48 · MCQ
- What is the criterion for DNA fragments movement on agarose gel during gel electrophoresis ?2017 · Q43 · MCQ
- DNA fragments are2017 · Q44 · MCQ
- A gene whose expression helps to identify transformed cell is known as2017 · Q45 · MCQ
- The process of separation and purification of expressed protein before marketing is called :2017 · Q46 · MCQ
- The DNA fragments separated on an agarose gel can be visualised after staining with2017 · Q54 · MCQ
- Which of the following is not a feature of the plasmids ?2016 · Q44 · MCQ
- Which of the following is a restriction endonuclease ?2016 · Q45 · MCQ
- Which of the following is not required for any of the techniques of DNA fingerprinting available at present?2016 · Q46 · MCQ
- The taq polymerase enzyme is obtained from2016 · Q55 · MCQ
- A foreign DNA and plasmid cut by the same restriction endonuclease can be joined to form a recombinant plasmid using2016 · Q41 · MCQ
- Which of the following is not a component of downstream processing ?2016 · Q42 · MCQ
- Which of the following restriction enzymes produces blunt ends ?2016 · Q43 · MCQ
- Stirred-tank bioreactors have been designed for -2016 · Q52 · MCQ
- The cutting of DNA at specific locations became possible with the discovery of -2015 · Q42 · MCQ
- The DNA molecule to which the gene of interest is integrated for cloning is called -2015 · Q68 · MCQ
- Which vector can clone only a small fragment of DNA ?2014 · Q41 · MCQ
- Commonly used vectors for human genome sequencing are :2014 · Q42 · MCQ
- An analysis of chromosomal DNA using the Southern hybridization technique does not use:2014 · Q51 · MCQ
- During the process of isolation of DNA, chilled ethanol is added to :2013 · Q75 · MCQ
- DNA fragments generated by the restriction endonucleases in a chemical reaction can be separated by :2013 · Q77 · MCQ
- Which of the following is not corretly matched for the organism and its cell wall degrading enzyme ?2013 · Q78 · MCQ
- The colonies of recombinat bacteria appear white in contrast to blue colonies of non-recombinant bacteria because of :2013 · Q79 · MCQ
- The figure below shows three steps (A, B, C) of Polymerase Chain Reaction (PCR). Select the option giving correct identification together with what it represents ? Includes diagram2012 · Q49 · MCQ
- In genetic engineering, the antibiotics are used2012 · Q50 · MCQ
- Which one of the following represents a palindromic sequence in DNA ?2012 · Q51 · MCQ
- Biolistics (gene-gun) is suitable for -2012 · Q52 · MCQ
- Which part would be most suitable for raising virus free plants for microporpagation ?2012 · Q84 · MCQ
- The figure below is the diagrammatic respesentation o the E.Coli vector pBR 322. Which one of the given options correctly identifies its certain component(s) ? Includes diagram2012 · Q90 · MCQ
- PCR and Restriction Fragment Length Polymorphism are the methods for2012 · Q91 · MCQ
- A single strand of nucleic acid tagged with a radioactive molecule is called2012 · Q92 · MCQ
- Which one is a true statement regarding DNA polymerase used in PCR ?2012 · Q93 · MCQ
- For transformation, micro - particles coated with DNA to be bombarded with gene are made up of2012 · Q94 · MCQ
- Which one of the following techniques made it possible to genetically engineer living organisms ?2011 · Q48 · MCQ
- Given below is a sample of a portion of DNA strand giving the base sequence on the opposite strands. What is so special shown in it ? 5' .............. GAATTC ................ 3' 3' .............. CTTAAG ................. 5'2011 · Q78 · MCQ
- Agarose extracted from sea weeds finds use in :2011 · Q79 · MCQ
- There is a restriction endonuclease called EcoRl. What does "co" part in it stand for ?2011 · Q80 · MCQ
- In genetic engineering, a DNA segment (gene) of interest,is transferred to the host cell through a vector. Consider the following four agents (A-D) in this regard and select the correct option about which one or more of these can be used…2010 · Q38 · MCQ
- Which one of the following is used as vector for cloning genes into higher organisms ?2010 · Q47 · MCQ
- Restriction endonucleases are enzymes which –2010 · Q48 · MCQ
- Which of the following are used in gene cloning ?2010 · Q49 · MCQ
- Stirred-tank bioreactors have been designed for –2010 · Q50 · MCQ
- DNA or RNA segment tagged with a radioactive moleculer is called –2010 · Q59 · MCQ
- Somaclones are obtained by : -2009 · Q80 · MCQ
- Which one of the following is commonly used in transfer of foreign DNA into crop plants ?2009 · Q83 · MCQ
- Gel electrophoresis is used for:2008 · Q83 · MCQ
- The linking of antibiotic resistance gene with the plasmid vector became possible with:2008 · Q84 · MCQ
- Restriction endonuclease -2006 · Q45 · MCQ
- Two microbes found to be very useful in genetic engineering are-2006 · Q46 · MCQ
- Restriction endonucleases :-2004 · Q39 · MCQ
- In transgenics expression of transgene in target tissue is determined by :-2004 · Q40 · MCQ
- In recent years, DNA sequences (nucleotide sequence) of mt-DNA and Y chromosomes were considered for the study of human evolution, because : -2003 · Q49 · MCQ
- Which one of the following bacteria has found extensive use in genetic engineering work in plants ?2003 · Q50 · MCQ
- Manipulation of DNA in genetic engineering became possible due to the discovery of : -2002 · Q50 · MCQ
- In lederberg's replica plating experiment what shall be used to obtain streptomycin resistant strain : -2001 · Q38 · MCQ
- A mutant strain of T4 – Bacteriophage, R-II, fails to lyse the E-Coli but when two strains RIIX and R-IIY are mixed then they lyse the E.Coli. What may be the possible reason : -2001 · Q46 · MCQ
- Which of the following cut the DNA from specific places ?2001 · Q48 · MCQ
- Maximum number of bases in plasmids discovered so far : -2001 · Q49 · MCQ
- The bacteria generally used for genetic engineering is :2000 · Q45 · MCQ
- Plasmid has been used as vector because :2000 · Q46 · MCQ