NEETBiologyBiotechnology Principles and ProcessesMCQ+4 / −1
Following are the steps involved in the process of PCR.
A. Annealing
B. Amplification (~1 billion times)
C. Denaturation
D. Treatment with Taq polymerase and deoxynucleotides
E. Extension
Choose the correct sequence of steps of PCR from the options given below :
- A
- B
- C
- D
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Correct answer: A
The Polymerase Chain Reaction (PCR) is a method used to amplify DNA. The correct sequence of steps involved in the process of PCR is:
- Denaturation: The double-stranded DNA is heated to separate it into two single strands.
- Annealing: The temperature is lowered to allow primers to attach to the single-stranded DNA.
- Extension: Taq polymerase extends the primers to form a new DNA strand.
- Amplification: This cycle of heating and cooling is repeated multiple times (~1 billion times) to amplify the DNA.
Therefore, the correct sequence of steps of PCR from the options given is:
Option A:
$$\mathrm{C} \rightarrow \mathrm{A} \rightarrow \mathrm{D} \rightarrow \mathrm{E} \rightarrow \mathrm{B}$$
This option correctly represents the sequence of steps in PCR where:
- $\mathrm{C}$ represents Denaturation.
- $\mathrm{A}$ represents Annealing.
- $\mathrm{D}$ represents Treatment with Taq polymerase and deoxynucleotides (which occurs during the Extension step).
- $\mathrm{E}$ represents Extension.
- $\mathrm{B}$ represents Amplification (~1 billion times).
Hence, Option A is the correct sequence.
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