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Biotechnology Principles and Processes question

2024 · Q90
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Biotechnology Principles and Processes question

2024 · Q90

NEETBiologyBiotechnology Principles and ProcessesMCQ+4 / −1

Following are the steps involved in the process of PCR.

A. Annealing

B. Amplification (~1 billion times)

C. Denaturation

D. Treatment with Taq polymerase and deoxynucleotides

E. Extension

Choose the correct sequence of steps of PCR from the options given below :

  1. A
    C→A→D→E→B\mathrm{C} \rightarrow \mathrm{A} \rightarrow \mathrm{D} \rightarrow \mathrm{E} \rightarrow \mathrm{B}C→A→D→E→B
  2. B
    A→B→E→D→C\mathrm{A} \rightarrow \mathrm{B} \rightarrow \mathrm{E} \rightarrow \mathrm{D} \rightarrow \mathrm{C}A→B→E→D→C
  3. C
    A→C→E→D→B\mathrm{A} \rightarrow \mathrm{C} \rightarrow \mathrm{E} \rightarrow \mathrm{D} \rightarrow \mathrm{B}A→C→E→D→B
  4. D
    D→B→E→C→A\mathrm{D} \rightarrow \mathrm{B} \rightarrow \mathrm{E} \rightarrow \mathrm{C} \rightarrow \mathrm{A}D→B→E→C→A
View written solutionFree

Correct answer: A

The Polymerase Chain Reaction (PCR) is a method used to amplify DNA. The correct sequence of steps involved in the process of PCR is:

  1. Denaturation: The double-stranded DNA is heated to separate it into two single strands.
  2. Annealing: The temperature is lowered to allow primers to attach to the single-stranded DNA.
  3. Extension: Taq polymerase extends the primers to form a new DNA strand.
  4. Amplification: This cycle of heating and cooling is repeated multiple times (~1 billion times) to amplify the DNA.

Therefore, the correct sequence of steps of PCR from the options given is:

Option A:

$$\mathrm{C} \rightarrow \mathrm{A} \rightarrow \mathrm{D} \rightarrow \mathrm{E} \rightarrow \mathrm{B}$$

This option correctly represents the sequence of steps in PCR where:

  1. $\mathrm{C}$ represents Denaturation.
  2. $\mathrm{A}$ represents Annealing.
  3. $\mathrm{D}$ represents Treatment with Taq polymerase and deoxynucleotides (which occurs during the Extension step).
  4. $\mathrm{E}$ represents Extension.
  5. $\mathrm{B}$ represents Amplification (~1 billion times).

Hence, Option A is the correct sequence.

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