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Biotechnology Principles and Processes question

2021 · Q58
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Biotechnology Principles and Processes question

2021 · Q58

NEETBiologyBiotechnology Principles and ProcessesMCQ+4 / −1
During the process of gene amplification using PCR, if very high temperature is not maintained in the beginning, then which of the following steps of PCR will be affected first?
  1. A
    Ligation
  2. B
    Annealing
  3. C
    Extension
  4. D
    Denaturation
View written solutionFree

Correct answer: D

∙\bullet∙ Option (d) is correct. Denaturation is first step of PCR that involves seperation of double-stranded DNA. The DNA is subjected to heating at high temperature (95∘^\circ∘C). This leads to breaking of hydrogen bonds between nucleotides and formation of single-stranded DNA. Thus, if high temperature is not maintained, denaturation will be affected.

∙\bullet∙ Ligation of DNA fragments is performed with the help of an enzyme called DNA ligase.

∙\bullet∙ Annealing is performed at 50∘^\circ∘-60∘^\circ∘C which is the second step that can get affected.

∙\bullet∙ Addition of nucleotides to the primer, synthesizing a new DNA strand using only the template sequences with the help of enzyme DNA polymerase is called primer extension/polymerisation.

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