Given below are two statements:
Statement I : Restriction endonucleases recognise specific sequence to cut DNA known as palindromic nucleotide sequence.
Statement II : Restriction endonucleases cut the DNA strand a little away from the centre of the palindromic site.
In the light of the above statements, choose the most appropriate answer from the options given below :
- ABoth Statement I and Statement II are correct
- BBoth Statement I and Statement II are incorrect
- CStatement I is correct but Statement II is incorrect
- DStatement I is incorrect but Statement II is correct
View written solutionFree
Correct answer: A
Option (a) is the correct answer because both the statements I and II are correct. Each restriction endonuclease recognises a specific palindromic nucleotide sequences in the DNA. It will bind to the DNA and cut each of the two strands of double helix at specific points. Restriction enzymes cut the strand of DNA a little away from the centre of the palindrome site; but between the same two bases on the opposite strands. So both the statements I and II are correct.
More from Biotechnology Principles and Processes
- Which of the following is not a desirable feature of a cloning vector?2022 · MCQ
- Match List-I with List-II . Choose the correct answer from the options given below. Includes table2021 · MCQ
- Which of the following is not an application of PCR (Polymerase Chain Reaction)?2021 · MCQ
- Which of the following is a correct sequence of steps in a PCR (Polymerase Chain Reaction)?2021 · MCQ
- Plasmid pBR322 has Pst I restriction enzyme site within gene ampR that confers ampicillin resistance. If this enzyme is used for inserting a gene for -galactoside production and the recombinant plasmid is inserted in an E.coli strain2021 · MCQ
- During the process of gene amplification using PCR, if very high temperature is not maintained in the beginning, then which of the following steps of PCR will be affected first?2021 · MCQ
- A specific recognition sequence identified by endonucleases to make cuts at specific positions within the DNA is :2021 · MCQ
- During the purification process for recombinant DNA technology, addition of chilled ethanol precipitates out :2021 · MCQ