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Biotechnology Principles and Processes question

2014 · Q42
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Biotechnology Principles and Processes question

2014 · Q42

NEETBiologyBiotechnology Principles and ProcessesMCQ+4 / −1
Commonly used vectors for human genome sequencing are :
  1. A
    BAC and YAC
  2. B
    T/A Cloning Vectors
  3. C
    T− DNA
  4. D
    Expression Vectors
View written solutionFree

Correct answer: A

Bacterial artificial chromosome (BAC) vectors are based on natural, extra-chromosomal plasmid of E. coli. BAC vector contains genes for replication and maintenance of the F-factor, a selectable marker and cloning site. These vectors can accommodate upto 300-350 kb of foreign DNA and are also being used in genome sequencing project. Yeast artificial chromosome (YAC) vectors are used to clone DNA fragments of more than 1Mb in size. Therefore, they have been exploited extensively in mapping the large genomes, e.g., in the Human Genome Project. These vectors contain the telomeric sequence, the centromere and the autonomously replicating sequence from yeast chromosomes.

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