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Biotechnology Principles and Processes question

2021 · Q30
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Biotechnology Principles and Processes question

2021 · Q30

NEETBiologyBiotechnology Principles and ProcessesMCQ+4 / −1
Which of the following is a correct sequence of steps in a PCR (Polymerase Chain Reaction)?
  1. A
    Annealing, Denaturation, Extension
  2. B
    Denaturation, Annealing, Extension
  3. C
    Denaturation, Extension, Annealing
  4. D
    Extension, Denaturation, Annealing
View written solutionFree

Correct answer: B

PCR stands for Polymerase Chain Reaction. It is a technique in which multiple copies of gene of interest is synthesised using two sets of primers and the enzyme DNA polymerase. The correct sequence of steps in a PCR (Polymerase Chain Reaction) are

Denaturation In which the double-stranded template DNA is heated at 95°C to separate it into two single strands.



Annealing In which the temperature is lowered to 50°C which enables the DNA primers to attach to the template DNA.

Extension/Extending In which the temperature is raised and the new strand of DNA is made by the taq polymerase enzyme.



These three stages are repeated 20-40 times, doubling the number of DNA copies each time.

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