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Biotechnology Principles and Processes question

2021 · Q9
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Biotechnology Principles and Processes question

2021 · Q9

NEETBiologyBiotechnology Principles and ProcessesMCQ+4 / −1
During the purification process for recombinant DNA technology, addition of chilled ethanol precipitates out :
  1. A
    Polysaccharides
  2. B
    RNA
  3. C
    DNA
  4. D
    Histones
View written solutionFree

Correct answer: C

Various enzymes like protease, RNase, etc. are added to break down substances like proteins, RNA, etc. Once all these substances are broken down, DNA is left which is precipitated out by adding chilled ethanol.

Ethanol has a lower dielectric constant than water, making it to promote ionic bond formation the Na+ (from the salt) and the PO−3 (from the DNA backbone), further, causing the DNA to precipitate.

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