Match List I with List II.
| List - I | List - II | ||
|---|---|---|---|
| (A) | Kanamycin | (I) | Delivers genes into animal cells |
| (B) | ClaI | (II) | Selectable marker |
| (C) | Disarmed retroviruses | (III) | Restriction site |
| (D) | Kanamycin gene | (IV) | Antibiotic resistance |
Choose the correct answer from the options given below :
- A(A)-(II), (B)-(III), (C)-(I), (D)-(IV)
- B(A)-(III), (B)-(I), (C)-(IV), (D)-(II)
- C(A)-(IV), (B)-(III), (C)-(I), (D)-(II)
- D(A)-(II), (B)-(IV), (C)-(I), (D)-(III)
View written solutionFree
Correct answer: A
The correct answer is Option A :
(A) Kanamycin - (II) Selectable marker : Kanamycin is an antibiotic, and a resistance gene for kanamycin is often used as a selectable marker in genetic engineering. Cells that have been successfully transformed with the desired DNA will also carry the kanamycin resistance gene, and thus will survive in a culture medium containing kanamycin, while cells without the gene will not.
(B) ClaI - (III) Restriction site : ClaI is a restriction enzyme used in molecular biology to cut DNA at specific sites (restriction sites).
(C) Disarmed retroviruses - (I) Delivers genes into animal cells : Retroviruses are often used as vectors in gene therapy to deliver genes into animal cells. They can be "disarmed" to make them safe for use in humans or other animals.
(D) Kanamycin $^\mathrm{R}$ gene - (IV) Antibiotic resistance : The kanamycin resistance (Kan^R) gene is a type of antibiotic resistance gene. It produces a protein that inactivates kanamycin, allowing bacteria containing this gene to survive in the presence of this antibiotic.
More from Biotechnology Principles and Processes
- Which of the following is not a cloning vector?2023 · MCQ
- Given below are two statements: one is labelled as Assertion (A) and the other is labelled as Reason (R). Assertion (A) : When a particular restriction enzyme cuts strand of DNA, overhanging stretches or sticky ends are formed. Reason (R)…2022 · MCQ
- Separation of DNA fragments is done by a technique known as2022 · MCQ
- The enzyme (a) is needed for isolating genetic material from plant cells and enzyme (b) for isolating genetic material from fungus. Choose the correct pair of options from the following :2022 · MCQ
- Match List-I with List-II : Choose the correct answer from the options given below: Includes table2022 · MCQ
- Pathogenic bacteria gain resistance to antibiotics due to changes in their :2022 · MCQ
- Which of the following methods is not commonly used for introducing foreign DNA into the plant cell?2022 · MCQ
- Refer to the following statements for agarose-gel electrophoresis: (a) Agarose is a natural polymer obtained from sea-weed. (b) The separation of DNA molecules in agarose-gel electrophoresis depends on the size of DNA. (c) The DNA migrates…2022 · MCQ