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Biotechnology Principles and Processes question

2013 · Q75
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Biotechnology Principles and Processes question

2013 · Q75

NEETBiologyBiotechnology Principles and ProcessesMCQ+4 / −1
During the process of isolation of DNA, chilled ethanol is added to :
  1. A
    Break open the cell to release DNA
  2. B
    Remove proteins such as histones
  3. C
    Facilitate action of restriction enzymes
  4. D
    Precipitate DNA
View written solutionFree

Correct answer: D

Ethanol is much less polar than water. Adding it to the solution disrupts the screening charges exerted by water. The electrical attraction between phosphate and any positive ions (Na+) present in solution becomes strong enough to form a stable ionic bond and DNA precipitates. Ethanol precipitation is a widely used technique to purify, or concentrate nucleic acid.

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