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Biomolecules question

2020 · 2 Sep · Shift 2 · Q6
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Biomolecules question

2020 · 2 Sep · Shift 2 · Q6

JEE MainChemistryBiomoleculesMCQ+4 / −1
The correct observation in the following reactions is : JEE Main 2020 (Online) 2nd September Evening Slot Chemistry - Biomolecules Question 127 English
  1. A
    Formation of blue colour
  2. B
    Formation of violet colour
  3. C
    Formation of red colour
  4. D
    Gives no colour
View written solutionFree

Correct answer: C

  1. The options given are typical colour observations for protein/amino acid tests:

    • Blue colour often indicates the original colour of copper(II) solution.
    • Violet colour is the biuret test for proteins/peptide bonds.
    • Red colour is associated with the ninhydrin test only in special cases like proline/hydroxyproline giving yellow, while most amino acids give purple/blue-violet; brick-red is not the standard protein test outcome.
    • No colour means negative test.
  2. In biomolecules questions of this type, the asked observation usually concerns the reaction of a protein with copper sulfate in alkaline medium.

  3. The biuret test is based on peptide bonds reacting with alkaline \ceCu2+\ce{Cu^{2+}}\ceCu2+ solution to give a violet-coloured complex.

  4. Therefore, the correct observation is: Formation of violet colour\text{Formation of violet colour}Formation of violet colour

  5. Hence, the correct option is B.

  6. Comparison with stored answer:

    • Derived answer: B
    • Stored correct answer: C
    • These do not match.
  7. So I disagree with the stored answer. The likely correct answer should be B, assuming the reaction shown is the standard biuret test for proteins/peptide linkage detection.

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