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Biotechnology and Its Applications question

2023 · Q38
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Biotechnology and Its Applications question

2023 · Q38

NEETBiologyBiotechnology and Its ApplicationsMCQ+4 / −1

Main steps in the formation of Recombinant DNA are given below. Arrange these steps in a correct sequence.

A. Insertion of recombinant DNA into the host cell

B. Cutting of DNA at specific location by restriction enzyme

C. Isolation of desired DNA fragment

D. Amplification of gene of interest using PCR

Choose the correct answer from the options given below :

  1. A
    C, A, B, D
  2. B
    C, B, D, A
  3. C
    B, D, A, C
  4. D
    B, C, D, A
View written solutionFree

Correct answer: D

The correct answer is option (D) because recombinant DNA technology involves several steps in specific sequence such as isolation of DNA, fragmentation of DNA by restriction endonucleases, isolation of desired DNA fragment, ligation of the DNA fragment into a vector, transferring the recombinant DNA into the host, culturing the host cells in a medium at large scale and extraction of the desired product.

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