Which one is the correct product of DNA dependent RNA polymerase to the given template?
3'TACATGGCAAATATCCATTCA5'
- A5'AUGUACCGUUUAUAGGUAAGU3'
- B5'AUGUAAAGUUUAUAGGUAAGU3'
- C5'AUGUACCGUUUAUAGGGAAGU3'
- D5'ATGTACCGTTTATAGGTAAGT3'
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Correct answer: A
DNA-dependent RNA polymerase is an enzyme responsible for transcribing DNA into RNA. During transcription, the RNA polymerase reads the template strand of DNA and synthesizes a complementary RNA strand. A key point in this process is that RNA polymerase builds RNA by replacing thymine (T) with uracil (U).
The provided DNA template is:
3'TACATGGCAAATATCCATTCA5'
To find the correct RNA sequence, we need to identify the complementary base for each base in the template strand while considering RNA bases. Remember, in RNA:
- A (Adenine) pairs with U (Uracil)
- T (Thymine) pairs with A (Adenine)
- C (Cytosine) pairs with G (Guanine)
- G (Guanine) pairs with C (Cytosine)
The complementary RNA sequence to the DNA template is generated as follows:
- 3'T --> 5'A
- 3'A --> 5'U
- 3'C --> 5'G
- 3'G --> 5'C
- 3'T --> 5'A
- 3'A --> 5'U
- 3'T --> 5'A
- 3'G --> 5'C
- 3'G --> 5'C
- 3'C --> 5'G
- 3'A --> 5'U
- 3'A --> 5'U
- 3'T --> 5'A
- 3'A --> 5'U
- 3'T --> 5'A
- 3'C --> 5'G
- 3'C --> 5'G
- 3'A --> 5'U
- 3'T --> 5'A
- 3'T --> 5'A
- 3'C --> 5'G'
This constructs the RNA sequence:
5'AUGUACCGUUUAUAGGUAAGU3'
Thus, Option A correctly represents the RNA sequence transcribed by DNA-dependent RNA polymerase from the given DNA template:
Option A: 5'AUGUACCGUUUAUAGGUAAGU3'
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