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Biomolecules question

2019 · 9 Apr · Shift 2 · Q10
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Biomolecules question

2019 · 9 Apr · Shift 2 · Q10

JEE MainChemistryBiomoleculesMCQ+4 / −1
The peptide that gives positive ceric ammonium nitrate and carbylamine tests is :
  1. A
    Ser-Lys
  2. B
    Asp-Gln
  3. C
    Gln-Asp
  4. D
    Lys-Asp
View written solutionFree

Correct answer: A

  1. Identify what each test detects

    • Ceric ammonium nitrate test gives a positive result for alcoholic , −OH-OH−OH groups.
    • Carbylamine test gives a positive result only for primary amines , (−NH2)( -NH_2 )(−NH2​).
  2. Check which amino acid side chains can respond

    In peptides, the terminal groups are:

    • N-terminus: usually a free amino group
    • C-terminus: usually a free carboxyl group

    Also, side chains may contain relevant functional groups:

    • Serine (Ser): side chain −CH2OH-CH_2OH−CH2​OH , ⇒\Rightarrow⇒ alcohol present
    • Lysine (Lys): side chain −(CH2)4NH2-(CH_2)_4NH_2−(CH2​)4​NH2​ , ⇒\Rightarrow⇒ primary amine present
    • Aspartic acid (Asp): side chain −CH2COOH-CH_2COOH−CH2​COOH , no alcohol, no primary amine side chain
    • Glutamine (Gln): side chain −CH2CH2CONH2-CH_2CH_2CONH_2−CH2​CH2​CONH2​ , amide, not a primary amine for carbylamine test
  3. Evaluate each option

    Option A: Ser-Lys

    • Ser provides an alcohol group , ⇒\Rightarrow⇒ positive ceric ammonium nitrate test
    • Lys provides a primary amino group in side chain , ⇒\Rightarrow⇒ positive carbylamine test
    • Hence, this peptide gives both tests positive.

    Option B: Asp-Gln

    • Asp has carboxylic acid side chain, no alcohol
    • Gln has amide side chain, not primary amine for carbylamine test
    • Does not satisfy both tests.

    Option C: Gln-Asp

    • Same functional groups as option B, only order changed
    • Does not satisfy both tests.

    Option D: Lys-Asp

    • Lys gives primary amine , ⇒\Rightarrow⇒ carbylamine positive
    • Asp has no alcohol group , ⇒\Rightarrow⇒ ceric ammonium nitrate negative
    • Does not satisfy both tests.
  4. Final conclusion

    The peptide that gives both positive ceric ammonium nitrate and carbylamine tests is: Ser-Lys\boxed{\text{Ser-Lys}}Ser-Lys​

  5. Comparison with stored correct answer

    Stored correct answer: A

    Derived answer: A

    So, the derived answer agrees with the stored correct answer.

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