NEETBiologyMolecular Basis of InheritanceMCQ+4 / −1
DNA strands on a gel stained with ethidium bromide when viewed under UV radiation, appear as
- ABright blue bands
- BYellow bands
- CBright orange bands
- DDark red bands
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Correct answer: C
To make the DNA visible in the gel, ethidium bromide is added to the gel solution and the buffer. This positively charged polycyclic aromatic compound binds to DNA by inserting itself between the basepairs (intercalation). The DNA fragments when exposed to ultraviolet light appear as orange colour bands, due to the large increase in fluorescence of the ethidium bromide upon binding to the DNA.
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