NEETBiologyBiotechnology and Its ApplicationsMCQ+4 / −1
Polyethylene glycol method is used for :
- ABiodiesel production
- BSeedless fruit production
- CEnergy production from sewage
- DGene transfer without a vector
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Correct answer: D
Direct gene transfer is the transfer of naked DNA into plant cells, but the presence of rigid plant cell wall acts as a barrier to uptake. Therefore, protoplasts are the favoured target for direct gene transfer. Polyethylene glycol mediated DNA uptake is a direct gene transfer method that utilizes the interaction between PEG, naked DNA, salts and the protoplast membrane to effect transport of the DNA into the cytoplasm.
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