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Biomolecules question

2025 · Shift 2 · Q16
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Biomolecules question

2025 · Shift 2 · Q16

JEE AdvancedChemistryBiomoleculesNumerical+4 / −1
A linear octasaccharide (molar mass =1024 g mol−1=1024 \mathrm{~g} \mathrm{~mol}^{-1}=1024 g mol−1) on complete hydrolysis produces three monosaccharides: ribose, 2-deoxyribose and glucose. The amount of 2-deoxyribose formed is 58.26%(w/w)58.26 \%(\mathrm{w} / \mathrm{w})58.26%(w/w) of the total amount of the monosaccharides produced in the hydrolyzed products. The number of ribose unit(s) present in one molecule of octasaccharide is \qquad. Use: Molar mass (\left(\right.( in g mol−1)\left.\mathrm{mol}^{-1}\right)mol−1): ribose =150,2=150,2=150,2-deoxyribose =134=134=134, glucose =180=180=180; Atomic mass (in amu): H=1,O=16\mathrm{H}=1, \mathrm{O}=16H=1,O=16
Numerical answer
View written solutionFree

Correct answer: 2

  1. Let the numbers of monosaccharide units be:

    • Ribose units =r= r=r
    • 2-deoxyribose units =d= d=d
    • Glucose units =g= g=g

    Since it is a linear octasaccharide, total number of units is r+d+g=8 .r+d+g=8 \, .r+d+g=8.

  2. Use the given mass percentage after complete hydrolysis

    On hydrolysis, the constituent monosaccharides are obtained as free sugars.

    Given molar masses:

    \quad M(\text{2-deoxyribose})=134, \quad M(\text{glucose})=180$$ Mass of 2-deoxyribose obtained from 1 mol octasaccharide: $$134d$$ Total mass of all monosaccharides obtained: $$150r+134d+180g$$ Given: $$\frac{134d}{150r+134d+180g}\times 100 = 58.26$$ Hence, $$\frac{134d}{150r+134d+180g}=0.5826$$
  3. Relate hydrolyzed mass to the original octasaccharide mass

    A linear octasaccharide has 888 monosaccharide units joined by 777 glycosidic bonds.

    Each bond formation removes one H2O\mathrm{H_2O}H2​O molecule.

    Therefore, mass of octasaccharide=sum of monosaccharide masses−7×18\text{mass of octasaccharide} = \text{sum of monosaccharide masses} - 7\times 18mass of octasaccharide=sum of monosaccharide masses−7×18

    Given octasaccharide molar mass =1024=1024=1024: 150r+134d+180g−126=1024150r+134d+180g - 126 = 1024150r+134d+180g−126=1024 150r+134d+180g=1150150r+134d+180g = 1150150r+134d+180g=1150

  4. Use the percentage condition

    134d1150=0.5826\frac{134d}{1150}=0.58261150134d​=0.5826

    So, 134d=1150×0.5826=669.99≈670134d = 1150\times 0.5826 = 669.99 \approx 670134d=1150×0.5826=669.99≈670

    Thus, d=670134=5d=\frac{670}{134}=5d=134670​=5

    So the number of 2-deoxyribose units is d=5d=5d=5

  5. Now use total units equation

    r+g+5=8r+g+5=8r+g+5=8 r+g=3r+g=3r+g=3

  6. Use total hydrolysis mass equation

    150r+134(5)+180g=1150150r+134(5)+180g=1150150r+134(5)+180g=1150 150r+670+180g=1150150r+670+180g=1150150r+670+180g=1150 150r+180g=480150r+180g=480150r+180g=480 Divide by 303030: 5r+6g=165r+6g=165r+6g=16

    Also, r+g=3r+g=3r+g=3

    Multiply by 555: 5r+5g=155r+5g=155r+5g=15

    Subtract from 5r+6g=165r+6g=165r+6g=16: g=1g=1g=1

    Therefore, r=3−1=2r=3-1=2r=3−1=2

  7. Final answer

    The number of ribose units present in one molecule of octasaccharide is 2\boxed{2}2​

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