F. Skoog's famous experimentation on plant tissue culture involved the proliferation of callus from internodal segments of the tobacco stem when auxin was supplied along with certain substances. The substances that supported callus proliferation included extracts from vascular tissues, coconut milk, and yeast extract. However, abscisic acid (ABA) did not support this proliferation. Hence, the correct answer to the question is Option C, Abscisic acid.

Let me explain why:

Auxins are a class of plant hormones that play a critical role in the growth and behavioral processes in a plant's life cycle. However, for the induction of callus (an unorganized mass of plant cells), auxin alone is often not sufficient. The addition of other substances can synergistically enhance its effect. This has been shown through Skoog’s observations with the following substances:

1. Extract of Vascular Tissues: Vascular tissues contain various nutrients and growth factors that can promote cell division and growth.

2. Coconut Milk: Rich in cytokinins, which promote cell division and cooperation with auxins enhances callus formation.

3. Yeast Extract: Contains vitamins and nutrients that can aid in the growth and development of plant tissues.

On the other hand,

4. Abscisic Acid (ABA): ABA is typically known for its role in inhibiting growth and promoting dormancy, not for promoting callus formation. It acts in contrast to auxins and cytokinins, often inhibiting processes like germination and cell division. Thus, it does not support callus proliferation and is not used for this purpose in Skoog’s experiments.

Therefore, the correct answer is:

Option C: Abscisic acid