The correct answer is Option B: Botanical garden. Here's why:

In-situ conservation refers to the conservation of species in their natural habitats. This involves protecting and managing the ecosystems where these species live.

Let's analyze each option:

Option A: Wild-life sanctuary - These are protected areas specifically designated for the conservation of wild animals. They are prime examples of in-situ conservation.

Option B: Botanical garden - Botanical gardens are cultivated areas where plants are collected, grown, and displayed for scientific research, conservation, and public education. While they play a crucial role in plant conservation, they are not natural habitats and therefore don't fall under in-situ conservation.

Option C: Biosphere reserve - These are areas designated by UNESCO where humans and nature coexist sustainably. They encompass a range of ecosystems, promoting both conservation and sustainable use, making them a form of in-situ conservation.

Option D: National park - National parks are large areas of land protected for their natural beauty, wildlife, and ecosystems. They are dedicated to preserving nature in its natural state, making them a key example of in-situ conservation.

Therefore, the only option not included under in-situ conservation is Option B: Botanical garden.