C₄ plants show kranz type of anatomy. In kranz anatomy, the mesophyll is undifferentiated and its cells occur in concentric layers around vascular bundles. The vascular bundles are surrounded by large sized bundle sheath cells which are arranged in wreath like manner in one to several layers. In C₄ plants there are two carboxylation reactions, first in mesophyll chloroplast and second in bundle sheath chloroplast. RuBP is present in bundle sheath chloroplasts where C₃ cycle takes place.